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STEMCELL Technologies Inc rosettesep tm human nk cell enrichment cocktail
Rosettesep Tm Human Nk Cell Enrichment Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosettesep+tm+human+nk+cell+enrichment+cocktail/rosettesep+human+b+cell+enrichment+cocktail/pmc12269682-250-8-15
Average 90 stars, based on 1 article reviews
rosettesep tm human nk cell enrichment cocktail - by Bioz Stars, 2026-09
90/100 stars

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Isolation:

Article Title: Tuning antibody stability and function by rational designs of framework mutations
Article Snippet: Optical absorbance was read on FLUOstar Omega spectrophotometer (BMG Labtech). .. Natural killer (NK) cells were isolated using the RosetteSep TM Human NK Cell Enrichment Cocktail (STEMCELL Technologies) following the manufacturer’s instructions. ..

Article Title: Hybrid IgE-IgG1 antibodies (IgEG): a new antibody class that combines IgE and IgG functionality
Article Snippet: Peripheral blood mononuclear cells (PBMCs) of healthy volunteers were isolated using Ficoll-Paque as previously described. .. Primary NK cells were isolated using the RosetteSep TM Human NK Cell Enrichment Cocktail (Stemcell) following the manufacturer’s instructions. ..

Article Title: An Anti-inflammatory microRNA Signature Distinguishes Group 3 Innate Lymphoid Cells From Natural Killer Cells in Human Decidua
Article Snippet: Cell suspensions from decidual and tonsil tissues were obtained with GentleMacs dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) as previously described ( ). .. Decidua infiltrating lymphocytes (DILs), tonsil lymphocytes and peripheral blood mononuclear cells from blood samples were isolated after density gradient centrifugation over Ficoll Lympholyte®-H Fycoll (Cederlane, Burlington, Canada). pbNK cells (purity >90% for all the samples; mean: 95%) were obtained with RosetteSep TM human NK cell enrichment cocktail (StemCell Technologies; Vancouver, Canada). ..

Article Title: Off-the-shelf CAR natural killer cells secreting IL-15 target spike in treating COVID-19
Article Snippet: .. NK cells were isolated by using the RosetteSep TM human NK cell enrichment cocktail (Cat# 15065, StemCell Technologies) and Ficoll-Paque (Cat# 17144003, Cytiva). .. The purity of primary NK cells was confirmed with flow cytometry using anti-CD56 (Cat# IM2474U, Beckman Coulter; 1:20 dilution) and anti-CD3 (Cat# 130-113-134, Miltenyi Biotec; 1:50 dilution) antibodies.

Article Title: Overexpression of PTPRN Promotes Metastasis of Lung Adenocarcinoma and Suppresses NK Cell Cytotoxicity
Article Snippet: .. Peripheral blood mononuclear cells were isolated by RosetteSep TM Human NK Cell Enrichment Cocktail (STEMCELL Technologies). .. We stained NK cells with 5 μL/test FITC-CD3 (Biolegend) and 5 μL/test PE-CD56 (Biolegend) at 4°C for 30 min. NK cells were washed with PBS and suspended in 400 μL of PBS after staining.

Incubation:

Article Title: An antibody with Fab-constant domains exchanged for a pair of C H 3 domains
Article Snippet: .. Venous blood was collected into citrate tubes (Beckton Dickinson) and incubated with RosetteSep TM Human NK Cell Enrichment Cocktail (Stemcell Technologies) for 20 min at RT. .. The mix was overlayed onto a Ficoll-Paque layer in Sep-Mate tubes (Stemcell technologies) and the NK cells were collected in the upper layer after centrifugation at 1200g for 10 min with brake on.

Gradient Centrifugation:

Article Title: An Anti-inflammatory microRNA Signature Distinguishes Group 3 Innate Lymphoid Cells From Natural Killer Cells in Human Decidua
Article Snippet: Cell suspensions from decidual and tonsil tissues were obtained with GentleMacs dissociator (Miltenyi Biotech, Bergisch Gladbach, Germany) as previously described ( ). .. Decidua infiltrating lymphocytes (DILs), tonsil lymphocytes and peripheral blood mononuclear cells from blood samples were isolated after density gradient centrifugation over Ficoll Lympholyte®-H Fycoll (Cederlane, Burlington, Canada). pbNK cells (purity >90% for all the samples; mean: 95%) were obtained with RosetteSep TM human NK cell enrichment cocktail (StemCell Technologies; Vancouver, Canada). ..



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